spheroid formation medium Search Results


dmem/f12 medium  (Thermo Fisher)
90
Thermo Fisher dmem/f12 medium
Dmem/F12 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spheroid formation  (PromoCell)
94
PromoCell spheroid formation
Spheroid Formation, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spheroid formation medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc spheroid formation medium
Spheroid Formation Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tumor spheroid formation medium cat#20141-500  (ProMab Inc)
90
ProMab Inc tumor spheroid formation medium cat#20141-500
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Tumor Spheroid Formation Medium Cat#20141 500, supplied by ProMab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spheroid formation medium b27  (Thermo Fisher)
90
Thermo Fisher spheroid formation medium b27
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Spheroid Formation Medium B27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stempro osteogenesis differentiation kit  (Thermo Fisher)
90
Thermo Fisher stempro osteogenesis differentiation kit
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Stempro Osteogenesis Differentiation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fbs ( n.b . the use of medium 199 as opposed to dmem was found to significantly enhance the formation and health of spheroids from both lines)  (Thermo Fisher)
90
Thermo Fisher fbs ( n.b . the use of medium 199 as opposed to dmem was found to significantly enhance the formation and health of spheroids from both lines)
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Fbs ( N.B . The Use Of Medium 199 As Opposed To Dmem Was Found To Significantly Enhance The Formation And Health Of Spheroids From Both Lines), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fbs ( n.b . the use of medium 199 as opposed to dmem was found to significantly enhance the formation and health of spheroids from both lines) - by Bioz Stars, 2026-03
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advanced dmem/f12  (PeproTech)
90
PeproTech advanced dmem/f12
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Advanced Dmem/F12, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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advanced dmem/f12 - by Bioz Stars, 2026-03
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dmem  (Millipore)
90
Millipore dmem
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Dmem, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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100 mm bacteriological grade petri dishes  (Thermo Fisher)
90
Thermo Fisher 100 mm bacteriological grade petri dishes
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
100 Mm Bacteriological Grade Petri Dishes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cultrex spheroid formation ecm sfm  (Trevigen)
90
Trevigen cultrex spheroid formation ecm sfm
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Cultrex Spheroid Formation Ecm Sfm, supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mammocult medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mammocult medium
The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
Mammocult Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid formation assay to assess the single-cell derived tumor-spheroid formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.

Journal: Frontiers in Cell and Developmental Biology

Article Title: PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer

doi: 10.3389/fcell.2022.798590

Figure Lengend Snippet: The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid formation assay to assess the single-cell derived tumor-spheroid formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.

Article Snippet: PCa cells were made in single-cell suspensions and 1 × 10 5 cells were cultured in suspension in 2 ml of tumor spheroid formation medium (Cat#20141-500, promab Cancer Stem PremiumTM).

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Control, Migration, Tube Formation Assay, Derivative Assay